![]() The proteins are placed onto blotting paper that’s made from a material such as nitrocellulose.Īn enzyme is added to the paper. The Western blot uses a procedure called gel electrophoresis to identify and separate proteins by molecular weight and length. Antigens stimulate your immune system to push out antibodies in an effort to fight the disease. If you develop a viral, fungal, or bacterial infection, your body will produce proteins called antigens in response. The Western blot test looks for the antibodies against an infection, not the infection itself. If you get a positive result for Lyme disease or HIV after taking an enzyme-linked immunosorbent assay (ELISA) test, your doctor may recommend a Western blot test to you. It was invented in 1979 to identify ribosomal RNA binding proteins. Appl Microbiol Biotechnol 102:8931–8942.The Western blot test is also known as a protein immunoblot test or immunoblotting. Yang S, Li L, Yin S et al (2018) Single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus. Plotzki E, Keller M, Ivanusic D, Denner J (2016) A new western blot assay for the detection of porcine cytomegalovirus (PCMV). Īmeri M, Zhou EM, Hsu WH (2006) Western blot immunoassay as a confirmatory test for the presence of anti-mycoplasma hyopneumoniae antibodies in swine serum. Hedman C, Otero A, Douet JY, Lacroux C, Lugan S, Filali H, Corbière F, Aron N, Badiola JJ, Andréoletti O, Bolea R (2018) Detection of PrPres in peripheral tissue in pigs with clinical disease induced by intracerebral challenge with sheep-passaged bovine spongiform encephalopathy agent. ![]() Greenlee JJ, Kunkle RA, Smith JD, Greenlee MHW (2016) Scrapie in swine: a diagnostic challenge. (87)90076-6īhatt M, Mohapatra JK, Pandey LK, Mohanty NN, Das B, Prusty BR, Pattnaik B (2018) Mutational analysis of foot and mouth disease virus nonstructural polyprotein 3AB-coding region to design a negative marker virus. ![]() Urzainqui A, Tabarés E, Carrasco L (1987) Proteins synthesized in African swine fever virus-infected cells analyzed by two-dimensional gel electrophoresis. Current topics in veterinary medicine and animal science, vol 29. In: McNulty MS, McFerran JB (eds) Recent advances in virus diagnosis. Todd D, McNulty MS, Allan GM (1984) The use of polyacrylamide gel electrophoresis of virus RNA in the study of rotavirus infections. Kattoor JJ, Saurabh S, Malik YS, Sircar S, Dhama K, Ghosh S, Bányai K, Kobayashi N, Singh RK (2017) Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene. Kurien BT, Scofield RH (2009) Protein blotting and detection: methods and protocols. Wilson K, Walker JM, Hofmann A, Clokie S (2018) Wilson and Walker’s principles and techniques of biochemistry and molecular biology. Kurien BT, Scofield RH (2015) Western blotting: an introduction. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD et al (1985) Measurement of protein using bicinchoninic acid. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. īradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. In: Encyclopedia of movement disorders, vol 2010. Academic, Boca Raton, pp 99–117Ĭounts SE (2010) Western blot. In: Basic science methods for clinical researchers. J Mol Biol 98(3):503–517Īlwine JC, Kemp DJ, Stark GR (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. Proc Natl Acad Sci U S A 76(9):4350īurnette WN (1981) “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |